Vitamin D3 Controls TLR4- and TLR2-Mediated Inflammatory Responses of Endometrial Cells

<b><i>Objectives:</i></b> Vitamin D has potent immunoregulatory features and modulates innate adaptive immune responses. There is a significant association between intrauterine infection-associated inflammatory responses pregnancy complications such as abortion preterm labor. Here, we investigated how 1,25 (OH)2 D3 could modulate of endometrial cells. <b><i>Design:</i></b> This an in vitro experimental study. Endometrial stromal cells (ESCs) whole (WECs) were collected from 15 apparently normal women, the immunomodulatory effects on lipopolysaccharide (LPS)- or lipoteichoic acid (LTA)-treated ESCs WECs investigated. <b><i>Participants/Materials, Setting, Methods:</i></b> Women with no history abortion, infertility, endometriosis, sign vaginal infection enrolled this samples by gynecologists using Pipelle pipette proliferative phase menstrual cycle. treated either LPS LTA. The levels IL-6, IL-8, TNF-α culture supernatants quantified ELISA technique. TLR2, TLR4, MyD88 expressions assessed RT-qPCR. TLR4 expression at protein level was studied Western blot <b><i>Results:</i></b> Dihydroxycholecalciferol (1,25 D3) significantly reduced production LPS-activated IL-6 LTA-stimulated WECs. In contrast, pretreatment increased IL-8 LPS- markedly LPS-induced ESCs. treatment induced gene expression. effect reversed when these pretreated before stimulation LPS. <b><i>Limitations:</i></b> Because small size samples, doing experiments all together some not feasible. Confirmation results obtained here needs well-designed vivo studies. <b><i>Conclusions:</i></b> molecule essential for maintaining homeostasis controlling potentially harmful associated female reproductive tract infections.